RXBio Translates Sequence to Science and Industry
When a single cell proliferates for more than 6 generations in vitro, the cell population composed of its descendants becomes a colony or clone. Each clone contains more than 50 cells and is between 0.3 – 1.0 mm in size. The colony formation rate indicates the independent survival ability of cells. Various physical and chemical factors may lead to changes in the clone formation ability of cells. The clone formation ability of cells can be detected through certain experimental methods.
The cell clone formation rate, that is, the cell seeding survival rate, represents the number of adherent cells that survive and form clones after cell seeding. Not every adherent cell can proliferate and form clones, while the cells that form clones must be adherent and have proliferative vitality. The clone formation rate reflects two important characteristics: cell population dependence and proliferative ability.
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