introduction

Untargeted metabolomics is a commonly used research method in metabolomics. The main research approach is to compare the experimental group with the control group, detect all metabolites in the samples, obtain quantitative information, and identify the differential metabolites and metabolic pathways between different groups. It can provide clues and directions for research on the development of disease biomarkers, the pathogenesis of diseases and the mechanisms of drug treatment.

advantages

✔ High throughput: Detect and analyze all metabolic signals in samples at one time.

✔ Wide coverage: Detect nearly a thousand metabolites, covering a rich variety of substance types.

✔ Low cost: The sample pretreatment is simple and the cost is relatively low.

✔ Accurate identification: Four methods including the high-resolution self-built library, the DB_all public library, the AI prediction library and MetDNA are adopted for identification.

Applications

✔ Look for biomarkers of diseases.
✔ Study the metabolic pathways related to the onset of diseases.
✔ Predict the efficacy and adverse reactions of drugs.
✔ Evaluate the efficacy and safety of traditional Chinese medicine.

Research Cases

Title：Supplementary Hesperidin Alleviated CPT-11-Induced Diarrhea by Modulating Gut Microbiota and Inhibiting the IL-17 Signaling Pathway

Journal：Journal of Agricultural and Food Chemistry（双一区，IF=5.7）

Publication Date：2025.3

DOI：10.1021/acs.jafc.4c09602

Irinotecan (CPT-11) is a chemotherapeutic drug commonly used in the treatment of gastrointestinal tumors, and diarrhea is a common adverse reaction. Hesperidin, a flavonoid abundant in citrus fruits, has shown potential in controlling CPT-11-induced diarrhea (CID). However, the mechanism behind its effect remains unclear. In this study, a CID mouse model was established by administering CPT-11, and the effects of hesperidin on the severity of diarrhea, intestinal pathology, composition of the intestinal microbiota, and metabolite profiles were evaluated through biochemical analysis, histopathology, immunohistochemistry, 16S rRNA sequencing, and untargeted metabolomics. In addition, transcriptomics analysis, molecular docking, and molecular dynamics simulations were also conducted to investigate the potential mechanism of action. It was found that hesperidin supplementation could significantly alleviate CID in mice. Analysis of the intestinal microbiota using 16S rRNA sequencing indicated that hesperidin improved the microbial composition, with key taxa such as Alistipes, Limosilactobacillus, Rikenella, and Mucispirillum playing a central role in improving CID. Moreover, hesperidin enhanced the intestinal barrier function by upregulating tight junction proteins, alleviating epithelial damage, and reducing the expression of IL-17A, TARF6, p38, phosphorylated p38 (P-p38), and AP-1 proteins in the colon. These findings suggest that hesperidin supplementation alleviates CID by regulating the intestinal microbiota and inhibiting the IL-17 signaling pathway, thereby improving the integrity of the intestinal barrier.

Reference

Zhang X, Yang Y, Wen M, et al. Supplementary Hesperidin Alleviated CPT-11-Induced Diarrhea by Modulating Gut Microbiota and Inhibiting the IL-17 Signaling Pathway. J Agric Food Chem. 2025;73(10):5915-5930. doi:10.1021/acs.jafc.4c09602